A study on rapid fractionation of the serum protein by salting out method rather than
expensive electrophoresis was performed with standard normal serum and 100 sera of
the normal persons as an evaluation of the usefulness in the screening or replacent of
the paper electrophoretic procedure.
The results were as followings:
1. The filtration procedure was less time consuming and more simple than surface
active agent, span ether, treatment in albumin fractionation.
2. The values obtained from the 100 sera of normal persons were 6.99±0.49gm% in
the total protein, 4.13±0.37 gm% in the albumin, 2.86±0.35gm% in the total globulin,
0.95±0.21gm% in the alpha globulin, 0.92±0.29gm% in the beta globulin, 0.99±0.24gm%
in the gamma globulin and 1.44±0.25 in the A-G ratio, while the standard normal
serum revealed 6.60gm% in the total protein, 3.80gm% in the albumin, 2.80 gm% in the
total globulin, 0.80gm% in the alpha globulin, 1.17 gm% in the beta globulin 0.93gm% in
the gamma globulin and 1.35 in the A-G ratio.
3. From the above results, it see%ed to be approximated to the values of the paper
electrophoresis of another authors.
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